β-Estradiol 3-benzoate is primarily used in hormone replacement therapy (HRT) to alleviate symptoms of menopause, such as hot flashes, vaginal dryness, and osteoporosis. It is also employed in the treatment of hypoestrogenism caused by hypogonadism, castration, or primary ovarian failure. In veterinary medicine, it is utilized to manage reproductive disorders in animals. Additionally, it is sometimes included in contraceptive formulations to regulate menstrual cycles and prevent pregnancy. The compound's esterification enhances its stability and prolongs its activity, making it a preferred choice for sustained-release formulations.