α-Sophorose monohydrate is primarily used in biochemical research as a substrate for studying enzyme activity, particularly glycosidases and glycosyltransferases. It serves as a model compound to investigate carbohydrate metabolism and enzymatic pathways. Additionally, it is employed in the development of biosensors for detecting specific enzymes or monitoring metabolic processes. In the food industry, it can be utilized as a precursor for synthesizing functional sweeteners or additives. Its role in studying glycobiology also contributes to advancements in understanding cell signaling and pathogen interactions.