This chemical is widely used in biochemical and biotechnological research as a versatile tool for protein labeling and detection. Its biotin moiety allows for strong binding to avidin or streptavidin, enabling its use in techniques such as ELISA, Western blotting, and immunofluorescence. The chelating groups make it suitable for binding metal ions, facilitating applications in metal affinity chromatography and metal-based imaging. Additionally, its water-soluble nature and stability make it ideal for use in aqueous environments, particularly in studies involving protein purification, labeling, and tracking in live cells. Its tripotassium salt form ensures enhanced solubility and compatibility with biological systems.