16,16-dimethyl Prostaglandin F2β is primarily utilized in medical and research settings due to its role as a prostaglandin analog. It is often employed in studies related to reproductive biology, particularly in understanding and managing processes such as ovulation, luteolysis, and parturition. Its ability to induce uterine contractions makes it valuable in obstetrics for managing postpartum hemorrhage. Additionally, it is used in veterinary medicine to regulate estrus cycles in livestock, enhancing breeding efficiency. In research, it serves as a tool to investigate the physiological and pathological roles of prostaglandins in various tissues and systems.