Used in biochemical research for studying bile acid metabolism and liver function. Acts as a reference compound in metabolic studies involving steroid transformations. Employed in the development and validation of analytical methods such as HPLC and mass spectrometry for detecting dihydroxy bile acids. Shows utility in investigating the role of bile acid derivatives in intestinal absorption and cholesterol regulation. Also explored for its potential in modulating nuclear receptors involved in detoxification and energy homeostasis.