L-β-Homolysine dihydrochloride is primarily utilized in biochemical research and pharmaceutical development. It serves as a key intermediate in the synthesis of peptides and amino acid derivatives, which are essential for studying protein structures and functions. This compound is also employed in the development of enzyme inhibitors and receptor modulators, aiding in the exploration of potential therapeutic agents. Additionally, it finds application in cell culture studies, where it helps in understanding cellular metabolism and amino acid transport mechanisms. Its role in advancing drug discovery and biochemical research makes it a valuable compound in scientific investigations.