Nω-Phospho-L-arginine lithium salt hydrate is widely used in biochemical research, particularly in studies involving enzyme kinetics and cellular energy metabolism. It serves as a high-energy phosphate donor in enzymatic reactions, making it valuable for investigating ATP-dependent processes. This compound is also utilized in the synthesis of phosphoproteins and as a substrate for arginine kinases, aiding in the understanding of muscle metabolism and energy transfer mechanisms. Additionally, it plays a role in studying nitric oxide synthase activity, contributing to research on cardiovascular and neurological systems. Its stability and solubility in aqueous solutions make it a preferred choice for in vitro experiments and assays.