DL-threo-β-Methylaspartic acid is primarily used in biochemical research as a substrate or inhibitor in enzymatic studies. It plays a significant role in understanding the mechanisms of enzymes involved in amino acid metabolism, particularly those that interact with aspartic acid derivatives. This compound is also utilized in the study of bacterial metabolism, as it can mimic natural substrates and help elucidate metabolic pathways. Additionally, it has applications in the development of enzyme inhibitors for potential therapeutic use, particularly in targeting specific enzymes associated with diseases. Its stereochemistry makes it valuable for exploring stereospecific enzymatic reactions and designing chiral molecules in medicinal chemistry.