Used in biological research and biotechnology for selective protein digestion, particularly cleaving peptide bonds at the carboxyl side of aromatic amino acids like tyrosine, tryptophan, and phenylalanine. Commonly applied in cell isolation and tissue dissociation procedures, especially in preparing single-cell suspensions from tissues. Employed in peptide mapping and protein structure studies due to its specificity. Also utilized in some therapeutic formulations for debriding wounds and reducing inflammation, although such uses are limited and carefully controlled. Frequently used in enzymatic assays to study protease activity and inhibition mechanisms.