Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively stains calcium deposits in tissues, making it valuable in identifying mineralization in bone sections, cartilage, and pathological calcifications. The solution is commonly applied in skeletal development studies, where it helps visualize calcium-rich areas under microscopy. In analytical chemistry, Alizarin Red S serves as an indicator for metal ion detection, particularly for aluminum, calcium, and fluoride ions in solution. It forms colored complexes with these ions, allowing for qualitative and quantitative analysis through spectrophotometric methods. It is also used in educational labs to demonstrate chelation and pH-dependent color changes, as its hue shifts from yellow to red to purple depending on the pH. Additionally, it has applications in the textile industry as a dye for fabrics, leveraging its strong affinity for cellulose fibers.