Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively stains calcium deposits in tissues, making it valuable in identifying mineralization in bone sections and detecting calcified areas in pathological samples. The solution is commonly used in osteogenesis studies to visualize calcium-rich regions in cell cultures, especially in research involving stem cell differentiation into bone cells. In analytical chemistry, Alizarin Red S serves as a colorimetric reagent for detecting and quantifying calcium ions in solution. It forms a red complex with calcium, allowing for visual or spectrophotometric measurement. This property is exploited in assays to assess calcium binding or deposition in various materials. It is also applied in textile and dye industries as a pH indicator and mordant dye for fabrics like wool and silk, where it produces red to violet shades depending on the mordant used. Additionally, it has been used in educational labs to demonstrate chelation and metal ion detection due to its clear color change in the presence of specific metal ions.