Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively stains calcium deposits in tissues, making it valuable in identifying mineralization in bone sections, cartilage, and calcified lesions. It is commonly used in assays to detect osteogenic differentiation of stem cells, where calcium-rich deposits formed by differentiated cells bind to the dye, producing a bright red color. In analytical chemistry, Alizarin Red S serves as a complexometric indicator in metal ion titrations, particularly for detecting calcium and other divalent cations. Its color change in the presence of specific metal ions makes it useful in qualitative and quantitative analysis. The solution is also employed in educational labs to demonstrate diffusion and membrane permeability due to its distinct color and solubility properties. Additionally, it has been used in textile and dye industries as a pH indicator and mordant dye for fabrics.