Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively stains calcium deposits in tissues, making it valuable in identifying mineralization in bone sections, cartilage, and pathological calcifications. The solution is commonly applied in studies involving osteogenesis, where it helps visualize calcium nodules formed by osteoblast-like cells in cell culture assays. In analytical chemistry, Alizarin Red S serves as a complexometric indicator in metal ion titrations, particularly for detecting calcium and other divalent cations. Its color change upon binding to metal ions allows for visual or spectrophotometric detection. It is also used in educational labs to demonstrate dyeing processes and acid-base indicators due to its pH-dependent color shifts. Additionally, the compound finds use in the textile industry as a mordant dye for coloring fabrics, especially in combination with metal salts to produce red and orange shades.