Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively binds to calcium deposits in tissues, making it a key tool in identifying and visualizing calcified areas in bone sections, cell cultures, and pathological samples. It is commonly used in osteogenic differentiation studies, where it helps confirm the presence of mineralized nodules formed by stem cells or osteoblasts. In analytical chemistry, Alizarin Red S serves as a complexometric indicator in metal ion titrations, particularly for calcium and other alkaline earth metals. Its color change in the presence of metal ions allows for visual detection of endpoint in chelation processes. The solution is also employed in material science for testing the durability and corrosion resistance of metals and alloys, where it detects trace metal ions leaching from surfaces. Additionally, it has been used in educational labs to demonstrate dyeing techniques and acid-base indicators due to its pH-dependent color shifts.