Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively binds to calcium deposits in tissues, making it valuable for identifying mineralization in bone sections, cartilage, and calcified tumors under microscopy. In cell culture studies, it is employed to detect calcium phosphate deposits produced by osteoblasts, aiding in the assessment of bone cell differentiation and mineralization activity. The solution is also used in analytical chemistry as a complexometric indicator in metal titrations, particularly for detecting calcium and other divalent metal ions. Its color change in the presence of metal ions makes it useful in qualitative and quantitative analysis. Additionally, Alizarin Red S serves in textile and dye industries as a pH indicator and a dye for fabrics, due to its color variation with pH. It has also found use in educational labs for demonstrating chelation and precipitation reactions.