Bromophenol red is widely used as a pH indicator in various laboratory and industrial applications. It is particularly useful in biochemical and molecular biology experiments to monitor pH changes in solutions, typically transitioning from yellow at pH 5.2 to purple at pH 6.8. This makes it valuable in cell culture media preparation, where maintaining a specific pH range is critical for cell growth and viability. Additionally, it is employed in electrophoresis to visualize the progress of DNA or protein separation. In environmental testing, bromophenol red helps assess the pH of water samples, ensuring compliance with regulatory standards. Its color-changing properties also make it a useful tool in educational settings for teaching acid-base chemistry principles.