Alizarin Red S is widely used as a biological stain, particularly in histology and cytology, to identify calcium deposits in tissues, making it valuable for studying bone formation and mineralization processes. It is also employed in the textile industry as a dye for fabrics, providing vibrant shades of red and orange. In analytical chemistry, it serves as a pH indicator, changing color in response to varying acidity levels. Additionally, it is utilized in environmental studies to detect metal ions in water samples due to its chelating properties. Its versatility makes it a significant compound in both scientific research and industrial applications.