Used in bioconjugation for click chemistry applications, particularly in labeling biomolecules such as peptides, proteins, and nucleic acids. The Boc (tert-butoxycarbonyl) group serves as a protecting group for the nitrogen, enabling controlled and site-specific reactions by preventing unwanted side reactions until deprotection.

The propargyl group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-functionalized probes, allowing site-specific attachment of fluorophores, biotin, or other detection tags.

The aminooxy group facilitates stable oxime ligation with carbonyl-containing molecules like aldehydes or ketones, useful in glycoprotein remodeling or carbohydrate labeling.

The PEG4 spacer enhances solubility in aqueous systems, reduces aggregation, and minimizes steric interference, improving reaction efficiency and binding accessibility.

Commonly employed in the development of bioconjugates for diagnostic assays, targeted drug delivery systems, and chemical biology research tools.